Moczarski M., Kołdras M., 1982. Properties of tench - Tinca tinca L. sperm and experiments with freezing it at -196°C. Acta Ichthyol. Piscat. 12 (2): 41-49.
Properties of tench sperm were studied by means of the following indices: sperm volume, spermatozoa concentration, total number of spermatozoa, spermatocrit, and sperm motility. A series of diluting media was tested in order to find optimal conditions for tench sperm freezing; phosphate buffer (BF) enriched by a 15% addition of ethylene glycol (EG) was found to be the most suitable diluting medium.